Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis
Braz. j. med. biol. res
; 50(1): e5594, 2017. graf
Article
ي En
| LILACS
| ID: biblio-839239
المكتبة المسؤولة:
BR1.1
ABSTRACT
We aimed to study the renal injury and hypertension induced by chronic intermittent hypoxia (CIH) and the protective effects mediated by angiotensin 1-7 [Ang(1-7)]. We randomly assigned 32 male Sprague-Dawley rats (body weight 180-200 g) to normoxia control, CIH, Ang(1-7)-treated normoxia, and Ang(1-7)-treated CIH groups. Systolic blood pressure (SBP) was monitored at the start and end of each week. Renal sympathetic nerve activity (RSNA) was recorded. CTGF and TGF-β were detected by immunohistochemistry and western blotting. Tissue parameters of oxidative stress were also determined. In addition, renal levels of interleukin-6, tumor necrosis factor-α, nitrotyrosine, and hypoxia-inducible factor-1α were determined by immunohistochemistry, immunoblotting, and ELISA. TUNEL assay results and cleaved caspase 3 and 12 were also determined. Ang(1-7) induced a reduction in SBP together with a restoration of RSNA in the rat model of CIH. Ang(1-7) treatment also suppressed the production of reactive oxygen species, reduced renal tissue inflammation, ameliorated mesangial expansion, and decreased renal fibrosis. Thus, Ang(1-7) treatment exerted renoprotective effects on CIH-induced renal injury and was associated with a reduction of oxidative stress, inflammation and fibrosis. Ang(1-7) might therefore represent a promising therapy for obstructive sleep apnea-related hypertension and renal injury.
Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Peptide Fragments
/
Angiotensin I
/
Oxidative Stress
/
Acute Kidney Injury
نوع الدراسة:
Prognostic_studies
المحددات:
Animals
اللغة:
En
مجلة:
Braz. j. med. biol. res
موضوع المجلة:
BIOLOGIA
/
MEDICINA
السنة:
2017
نوع:
Article
/
Project document