Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India
Braz. J. Pharm. Sci. (Online)
; 53(4): e17006, 2017. tab, graf
Article
ي En
| LILACS
| ID: biblio-889417
المكتبة المسؤولة:
BR40.1
الموقع: BR40.1
ABSTRACT
ABSTRACT Dioscorea pentaphylla L., a wild tuber is used both as food and medicines among different ethnic groups of Similipal Biosphere Reserve, India. Tubers are used against skin infections. In order to establish and confirm tribal claims, methanol extract was subjected to fractionation. The active fraction (DP1) was subsequently used for further purification and NMR (Nuclear magnetic resonance) characterization. The phytochemical analysis revealed the presence of saponin groups. The antibacterial activity of DP1 was done against selected bacterial strains (Salmonella typhi, Shigella flexneri, Streptococcus pyogenes, Streptococcus mutans and Vibrio cholerae) using DD (disc diffusion), AWD (agar well diffusion) and broth dilution assay. The activity was compared with antibiotics Penicillin and Kanamycin. It was observed that DP1 showed significant inhibitory activity against the tested bacteria. The characterization of DP1 through NMR analysis and presence of proton in carbon position at C-3, C-19, C-18, C-21 and C-27 was same as the known compound "Diosgenin". Therefore, isolated compound was confirmed to be Diosgenin. The study for the first time showed that, diosgenin present in D. pentaphylla tuber was responsible for antibacterial and antioxidant potential. Present study highlights the importance of Dioscorea species as sources of diverse secondary metabolites for the isolation of active compound(s).
Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Dioscorea
/
India
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
En
مجلة:
Braz. J. Pharm. Sci. (Online)
موضوع المجلة:
Farmacologia
/
Teraputica
/
Toxicologia
السنة:
2017
نوع:
Article