Interleukin-4 protects from chemotherapy-induced peripheral neuropathy in mice modal via the stimulation of IL-4/STAT6 signaling
Acta cir. bras
; 33(6): 491-498, June 2018. graf
Article
ي En
| LILACS
| ID: biblio-949359
المكتبة المسؤولة:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the possible role of IL-4 signaling pathway in vincristine-induced peripheral neuropathy.Methods:
The mouse model of vincristine-induced peripheral neuropathy and interleukin (IL)-4 knockout mice were utilized to investigate the possible role of IL-4 signaling pathway in vincristine-induced peripheral neuropathy. Vincristine induced increased sensitivity to mechanical stimulation was measured by von Frey hair test 7 and 14 days after intraperitoneal administration of 0.1 mg/kg vincristine in mice. Relative expression levels of cytokines were detected by quantitative real-time PCR. STAT6 expression following vincristine treatment was assessed with western blotting.Results:
We discovered that IL-4/STAT6 signaling was down-regulated in vincristine-treated mice. Deletion of IL-4 in mice increased the sensitivity to mechanical allodynia. IL-4 knockout mice also produced more pro-inflammatory cytokines, including IL-1β and TNF-α. Notably, co-administration of exogenous recombination IL-4 significantly prevented vincristine-induced mechanical allodynia.Conclusion:
Anti-inflammatory cytokine IL-4 protects rodent model from vincristine-induced peripheral neuropathy via the stimulation of IL-4/STAT6 signaling and inhibition of the pro-inflammatory cytokines.Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Vincristine
/
Interleukin-4
/
Peripheral Nervous System Diseases
/
STAT6 Transcription Factor
/
Anti-Inflammatory Agents
/
Antineoplastic Agents, Phytogenic
نوع الدراسة:
Evaluation_studies
/
Prognostic_studies
المحددات:
Animals
اللغة:
En
مجلة:
Acta cir. bras
موضوع المجلة:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
السنة:
2018
نوع:
Article