Effects of cyclosporin, nifedipine and phenytoin on gingival myofibroblast transdifferentiation in monkeys
J. appl. oral sci
; 27: e20180135, 2019. graf
Article
ي En
| LILACS, BBO
| ID: biblio-975900
المكتبة المسؤولة:
BR1.1
ABSTRACT
Abstract Objective:
Myofibroblasts have been associated with the development of several pathologic fibrotic conditions. This longitudinal study aims to assess the proliferative and antiapoptotic effects of cyclosporin, nifedipine and phenytoin on gingival connective tissue cells of nonhuman primate, as well as to analyze a possible role of myofibroblasts in gingival overgrowth. Materials andMethods:
Gingival samples from the right superior canine area were obtained from 12 male monkeys ( Sapajus spp ) to comprise the control group. After one week, the animals were randomly assigned to three groups, which received daily oral doses of cyclosporin, nifedipine or phenytoin for 120 days. Gingival samples were collected from the left superior canine area of two animals of each group at 52 and 120 days. Histological sections were stained with hematoxylin and eosin, and immunoreacted against α-SMA, Ki- 67 and bcl-2.Results:
α-SMA immunoreaction was negative in the control and experimental groups. Similarly, no difference between groups concerning immunostaining against Ki-67 and bcl-2 was observed in connective tissue cells.Conclusion:
Based on this methodology, it may be concluded that gingival overgrowths induced by cyclosporin, nifedipine and phenytoin are not associated with neither myofibroblast transdifferentiation, proliferation nor apoptosis of gingival connective cells in monkeys.Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Phenytoin
/
Nifedipine
/
Cyclosporine
/
Cell Transdifferentiation
/
Myofibroblasts
/
Gingiva
نوع الدراسة:
Observational_studies
/
Risk_factors_studies
المحددات:
Animals
اللغة:
En
مجلة:
J. appl. oral sci
موضوع المجلة:
ODONTOLOGIA
السنة:
2019
نوع:
Article