Development and use a novel combined in-vivo and in-vitro assay for anti-inflammatory and immunosuppressive agents
IJPR-Iranian Journal of Pharmaceutical Research. 2013; 12 (3): 445-455
ي En
| IMEMR
| ID: emr-138301
المكتبة المسؤولة:
EMRO
ABSTRACT
Contact hypersensitivity [CHS] mouse model induced by 2, 4-dinitrofluorobenzene [DNFB] is thought to be a T helper 1 [Th1]-dominant response and used for investigating anti-inflammatory and immunosuppressive agents. However, it is hardly used for screening large-scale drugs because of the large number of animals and complex mechanisms involved in-vivo. In this study, we focused on whether T lymphocytes from CHS mouse model could maintain the state of immune response in-vitro and explored a suitable time for drugs screening. The results showed that CD4[+] T cells of CHS mice were higher compared with those in normal group. The expression of T-bet and GATA3 showed a Th1 shift and the levels of interleukin [IL]-2 and IL-4 also showed similar trend. Furthermore, IL-6 produced by T lymphocytes from CHS mice had a high level too. Then, we detected the effects of dexamethasone [DEX], cyclosporine A [CsA] and mycophenolate mofetil [MMF] on T lymphocytes in-vitro, and the data displayed that these immunosuppressive drugs could all inhibit the proliferation of T lymphocytes significantly. These findings suggested that T lymphocytes from CHS mice could mimic a similar immune response in-vitro, and it's also a suitable method for screening anti-inflammatory and immunosuppressive agents
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الفهرس:
IMEMR
الموضوع الرئيسي:
Interleukins
/
Dermatitis, Contact
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Dinitrofluorobenzene
/
Hypersensitivity
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Immunosuppressive Agents
/
Mice
/
Anti-Inflammatory Agents
المحددات:
Animals
اللغة:
En
مجلة:
Iran. J. Pharm. Res.
السنة:
2013