Improved prolactin crop-sac bioassay applying a morphometric approach
Microsc. electron. biol. celular
; 14(2): 139-46, 1990.
Article
ي En
| LILACS
| ID: lil-121627
المكتبة المسؤولة:
BR1.1
RESUMO
A pigeon crop-sac bioassay for prolactin was developed applying a morphometric approach for quantification of the epithelial cell proliferation induced by the hormone. A small male dove, indigenous from South America (Zenaida auriculata) previously castrated was used as experimental animal. The total dose of prolactin was divided into 4 daily injections administered systemically into the pectoral muscles. The number of the proliferated cell layers ysed as the bioassay end point was analyzedstatistically. This procedure yields a highly sensitive bioassay with an excellent precision index, particularly well suited for quantification of the different molecular forms of pituitary PRL
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الفهرس:
LILACS
الموضوع الرئيسي:
Columbidae
/
Prolactin
/
Biological Assay
/
Crop, Avian
المحددات:
Animals
اللغة:
En
مجلة:
Microsc. electron. biol. celular
السنة:
1990
نوع:
Article