Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
Rev. Soc. Bras. Med. Trop
; 39(2): 159-162, mar.-abr. 2006. ilus, tab
Article
ي En
| LILACS, SES-SP
| ID: lil-426908
المكتبة المسؤولة:
BR1.1
ABSTRACT
A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100 percent agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Rabies
/
Rabies virus
/
DNA, Viral
/
Digoxigenin
/
Nucleoproteins
نوع الدراسة:
Diagnostic_studies
المحددات:
Animals
/
Humans
اللغة:
En
مجلة:
Rev. Soc. Bras. Med. Trop
موضوع المجلة:
MEDICINA TROPICAL
السنة:
2006
نوع:
Article