Insulin 3-like hormone and its role in epididymo-testicular descent
Int. braz. j. urol
; 33(3): 407-413, May-June 2007. ilus
Article
ي En
| LILACS
| ID: lil-459864
المكتبة المسؤولة:
BR1.1
ABSTRACT
PURPOSE:
The role of insulin 3-like (Insl3) hormone signaling in the testicular descent process has been demonstrated. The purpose of the present study was to evaluate epididymal development in Insl3-deficient mice. MATERIALS ANDMETHODS:
Heterozygous and homozygous Insl3 mutants of a mixed CD1 X 129/Sv genetic background were generated by breeding Insl3-/- females with Insl3+/- males, and their genotypes were determined by polymerase chain reaction. On the first postnatal day, newborn males were sacrificed, embedded in paraffin, and cut in 4 µm sections. Sections were stained with hematoxylin/eosin and immunoreacted with anti-± actin antibody.RESULTS:
An analysis of stained sections indicated an arrest in the development of the epididymis in all homozygous mice. The cauda and corpus of the epididymis were undersized. Compared to the heterozygous epididymis, the homozygous epididymis had fewer peritubular layers and dwarfish musculature. We confirmed this with immunostaining with monoclonal antibodies against ± -smooth muscle actin.CONCLUSION:
Defective development of the smooth musculature in the epididymis of Insl3 homozygous mutant mice, combined with its high intraabdominal undescended position, supports previous observations regarding the importance of intact epididymis morphology and function for descent of the epididymo-testicular unit.Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Testis
/
Epididymis
/
Insulin
المحددات:
Animals
اللغة:
En
مجلة:
Int. braz. j. urol
موضوع المجلة:
UROLOGIA
السنة:
2007
نوع:
Article