Perfil citológico dos líquidos amniótico e alantoidiano nos terços inicial, médio e final da gestação na vaca / Cytology of amniotic and allantoic fluids in the cow at the first, second, and third trimester of pregnancy

ABSTRACT

The cytology of extraembrionic fluids in cows in the first, second, and third trimester of pregnancy was evaluated. For each trimester, 10 pregnant uteri, collected in the slaughterhouse were used. A volume of 20mL of amniotic and allantoic fluids was collected, and centrifuged at 200g for 10 minutes. The sediment was taken and processed on a slide for cytology examination after staining. The results showed that the total cells number of the amniotic fluid increased during gestation, with the panoptic staining method being efficient to evaluate the amniotic fluid cytology in crossbreed Holstein x Zebu cows.