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Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
Ferreira, Gabriella Freitas; Moraes, Cícero; Silveira, Alda Maria Soares da; Correa-Oliveira, Rodrigo; Teixeira-Carvalho, Andréa; Martins-Filho, Olindo Assis; Moreno, Elizabeth Castro; Carmo, Luiz Simeão do; Fraga, Lucia Alves de Oliveira; Malaquias, Luiz Cosme Cotta.
Affiliation
  • Ferreira, Gabriella Freitas; Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares. BR
  • Moraes, Cícero; Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares. BR
  • Silveira, Alda Maria Soares da; Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares. BR
  • Correa-Oliveira, Rodrigo; Instituto René Rachou-Fiocruz. Belo Horizonte. BR
  • Teixeira-Carvalho, Andréa; Instituto René Rachou-Fiocruz. Belo Horizonte. BR
  • Martins-Filho, Olindo Assis; Instituto René Rachou-Fiocruz. Belo Horizonte. BR
  • Moreno, Elizabeth Castro; Fundação Hemominas. Belo Horizonte. BR
  • Carmo, Luiz Simeão do; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia e Microbiologia. Belo Horizonte. BR
  • Fraga, Lucia Alves de Oliveira; Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares. BR
  • Malaquias, Luiz Cosme Cotta; Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares. BR
Mem. Inst. Oswaldo Cruz ; 107(3): 348-355, May 2012. graf
Article ي En | LILACS | ID: lil-624016
المكتبة المسؤولة: BR1.1
ABSTRACT
We investigated the cytokine profile of peripheral mononuclear cells from chronic osteomyelitis (OST) patients following in vitro stimulation with staphylococcal enterotoxin A (SEA). We demonstrate that stimulation with SEA induced prominent lymphocyte proliferation and high levels of tumour necrosis factor (TNF)-α, interleukin (IL)-4 and IL-10 secretion in both OST and non-infected individuals (NI). Even though stimulation with SEA had no impact on IL-6 production in either patient group, the baseline level of IL-6 production by cells from OST patients was always significantly less than that produced by cells from NI. After classifying the osteomyelitic episodes based on the time after the last reactivation event as "early" (1-4 months) or "late" osteomyelitis (5-12 months), we found that increased levels of TNF-α and IL-4 in combination with decreased levels of IL-6 were observed in the early episodes. By contrast, increased levels of IL-10, IL-2 and IL-6 were hallmarks of late episodes. Our data demonstrate that early osteomyelitic episodes are accompanied by an increased frequency of "high producers" of TNF-α and IL-4, whereas late events are characterised by increased frequencies of "high producers" of IL-10, IL-6 and IL-2. These findings demonstrate the distinct cytokine profiles in chronic osteomyelitis, with a distinct regulation of IL-6 production during early and late episodes.
الموضوعات
Key words

النص الكامل: 1 الفهرس: LILACS الموضوع الرئيسي: Osteomyelitis / Staphylococcal Infections / Staphylococcus aureus / Leukocytes, Mononuclear / Cytokines / Enterotoxins / Nitric Oxide نوع الدراسة: Observational_studies المحددات: Adolescent / Adult / Child / Female / Humans / Male اللغة: En مجلة: Mem. Inst. Oswaldo Cruz موضوع المجلة: MEDICINA TROPICAL / PARASITOLOGIA السنة: 2012 نوع: Article

النص الكامل: 1 الفهرس: LILACS الموضوع الرئيسي: Osteomyelitis / Staphylococcal Infections / Staphylococcus aureus / Leukocytes, Mononuclear / Cytokines / Enterotoxins / Nitric Oxide نوع الدراسة: Observational_studies المحددات: Adolescent / Adult / Child / Female / Humans / Male اللغة: En مجلة: Mem. Inst. Oswaldo Cruz موضوع المجلة: MEDICINA TROPICAL / PARASITOLOGIA السنة: 2012 نوع: Article