Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes
Braz. j. microbiol
; Braz. j. microbiol;43(2): 552-559, Apr.-June 2012. ilus, tab
Article
ي En
| LILACS
| ID: lil-644470
المكتبة المسؤولة:
BR32.1
ABSTRACT
Fourteen isolates of Corynebacteruim pseudotuberculosis of them 7 were isolated from sheep with Caseous Lymphadenitis "biotype 1" and 7 isolated from buffaloes with Oedematous Skin Disease "biotype 2". All isolates were identified by standard microbiological techniques and by polymerase chain reaction targeting, 16S rRNA and phospholipase D genes. Synergistic haemolytic titers of all isolates were assayed by plate technique. The presences of phospholipase D gene in supernatants of all isolates were performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot technique by using hyperimmune serum raised in rabbit immunized with recombinant phospholipase D gene antigen. The concentration of phospholipase D gene was assayed by scanning the bound phospholipase D gene with specific antibodies that appeared at 31.5 kDa. Results presented that there is no correlation between titer of Synergistic haemolytic activity and the actual phospholipase D genes concentration in culture supernatants. Also results presented that Synergistic haemolytic activity and phospholipase D genes produced by biotype 2 (buffalo isolates) was generally higher than those by biotype 1(sheep isolates).
Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Phospholipase D
/
In Vitro Techniques
/
RNA
/
Gene Expression Regulation
/
Corynebacterium pseudotuberculosis
/
Corynebacterium Infections
/
Lymphadenitis
المحددات:
Animals
اللغة:
En
مجلة:
Braz. j. microbiol
موضوع المجلة:
MICROBIOLOGIA
السنة:
2012
نوع:
Article