Structural alterations of foreskin caused by chronic smoking may explain high levels of urethral reconstruction failure using foreskin flaps
Int. braz. j. urol
; 38(4): 529-535, July-Aug. 2012. ilus, tab
Article
ي En
| LILACS
| ID: lil-649447
المكتبة المسؤولة:
BR1.1
ABSTRACT
OBJECTIVES:
The aim of the present study was to perform a stereological and biochemical analysis of the foreskin of smoker subjects. MATERIALS ANDMETHODS:
Foreskin samples were obtained from 20 young adults (mean = 27.2 years old) submitted to circumcision. Of the patients analyzed, one group (n = 10) had previous history of chronic smoking (a half pack to 3 packs per day for 3 to 13 years (mean = 5.8 ± 3.2). The control group included 10 nonsmoking patients. Masson's trichrome stain was used to quantify the foreskin vascular density. Weigert’s resorcin-fucsin stain was used to assess the elastic system fibers and Picrosirius red stain was applied to study the collagen. Stereological analysis was performed using the Image J software to determine the volumetric densities. For biochemical analysis, the total collagen was determined as µg of hydroxyproline per mg of dry tissue. Means were compared using the unpaired t-test (p < 0.05).RESULTS:
Elastic system fibers of smokers was 42.5% higher than in the control group (p = 0.002). In contrast, smooth muscle fibers (p = 0.42) and vascular density (p = 0.16) did not show any significant variation. Qualitative analysis using Picrosirius red stain with polarized light evidenced the presence of type I and III collagen in the foreskin tissue, without significant difference between the groups. Total collagen concentration also did not differ significantly between smokers and non-smokers (73.1µg/mg ± 8.0 vs. 69.2µg/mg ± 5.9, respectively, p = 0.23).CONCLUSIONS:
The foreskin tissue of smoking patients had a significant increase of elastic system fibers. Elastic fibers play an important role in this tissue’s turnover and this high concentration in smokers possibly causes high extensibility of the foreskin. The structural alterations in smokers’ foreskins could possibly explain the poor results in smoking patients submitted to foreskin fasciocutaneous flaps in urethral reconstruction surgery.Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Surgical Flaps
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Urethra
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Smoking
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Plastic Surgery Procedures
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Foreskin
نوع الدراسة:
Qualitative_research
المحددات:
Adult
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Humans
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Male
اللغة:
En
مجلة:
Int. braz. j. urol
موضوع المجلة:
UROLOGIA
السنة:
2012
نوع:
Article