ERKs and mitochondria-related pathways are essential for glycyrrhizic acid-mediated neuroprotection against glutamate-induced toxicity in differentiated PC12 cells
Braz. j. med. biol. res
; 47(9): 773-779, 09/2014. graf
Article
ي En
| LILACS
| ID: lil-719311
المكتبة المسؤولة:
BR1.1
ABSTRACT
The present study focuses on the neuroprotective effect of glycyrrhizic acid (GA, a major compound separated from Glycyrrhiza Radix, which is a crude Chinese traditional drug) against glutamate-induced cytotoxicity in differentiated PC12 (DPC12) cells. The results showed that GA treatment improved cell viability and ameliorated abnormal glutamate-induced alterations in mitochondria in DPC12 cells. GA reversed glutamate-suppressed B-cell lymphoma 2 levels, inhibited glutamate-enhanced expressions of Bax and cleaved caspase 3, and reduced cytochrome C (Cyto C) release. Exposure to glutamate strongly inhibited phosphorylation of AKT (protein kinase B) and extracellular signal-regulated kinases (ERKs); however, GA pretreatment enhanced activation of ERKs but not AKT. The presence of PD98059 (a mitogen-activated protein/extracellular signal-regulated kinase kinase [MEK] inhibitor) but not LY294002 (a phosphoinositide 3-kinase [PI3K] inhibitor) diminished the potency of GA for improving viability of glutamate-exposed DPC12 cells. These results indicated that ERKs and mitochondria-related pathways are essential for the neuroprotective effect of GA against glutamate-induced toxicity in DPC12 cells. The present study provides experimental evidence supporting GA as a potential therapeutic agent for use in the treatment of neurodegenerative diseases.
Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Signal Transduction
/
Neuroprotective Agents
/
Glutamic Acid
/
Glycyrrhizic Acid
/
Anti-Inflammatory Agents
المحددات:
Animals
اللغة:
En
مجلة:
Braz. j. med. biol. res
موضوع المجلة:
BIOLOGIA
/
MEDICINA
السنة:
2014
نوع:
Article
/
Project document