Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients
Acta cir. bras
; 29(supl.3): 39-43, 2014. graf
Article
ي En
| LILACS
| ID: lil-726244
المكتبة المسؤولة:
BR1.1
ABSTRACT
PURPOSE:
To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples.METHODS:
Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR.RESULTS:
In fibroblasts, KGF gene expression was 220±80 and 33.33±6.67 (M±SD; N=4), respectively for major and minor burn groups. In keratinocytes, KGF gene expression was 11.2±1.9 and 3.45±0.37 (M±SD; N=4), respectively for major and minor burn groups. In fibroblasts, HBD-4 gene expression was 15.0±4.0 and 11.5±0.5 (M±SD; N=4), respectively for major and minor burn. In keratinocyte, HBD-4 gene expression was 0.0±0.0 and 13.4±4.8 (M±SD; N=4), respectively for major and minor burn.CONCLUSIONS:
KGF expression was increased in burn patient fibroblasts compared to control group. In keratinocytes culture, KGF suppression is inversely proportional to burn extension; it is active and increased in major burn but decreased in minor burn. HBD-4 expression was increased in fibroblasts and decreased in keratinocytes from all burned patients. .Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Burns
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Keratinocytes
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Beta-Defensins
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Fibroblasts
نوع الدراسة:
Clinical_trials
المحددات:
Female
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Humans
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Male
اللغة:
En
مجلة:
Acta cir. bras
موضوع المجلة:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
السنة:
2014
نوع:
Article
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Project document