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Antiviral and myocyte protective effects of IL-28A in coxsackievirus B3-induced myocarditis
Wang, Shihong; Huang, Xingyuan; Zhang, Jing; Huang, Congxin.
Affiliation
  • Wang, Shihong; Wuhan University. Renmin Hospital of Wuhan University. Department of Pediatrics. Hubei. CN
  • Huang, Xingyuan; Wuhan University. Renmin Hospital of Wuhan University. Department of Pediatrics. Hubei. CN
  • Zhang, Jing; Wuhan University. Renmin Hospital of Wuhan University. Department of Pediatrics. Hubei. CN
  • Huang, Congxin; Wuhan University. Renmin Hospital of Wuhan University. Department of Pediatrics. Hubei. CN
Braz. j. infect. dis ; Braz. j. infect. dis;19(2): 132-140, Mar-Apr/2015. graf
Article ي En | LILACS | ID: lil-746517
المكتبة المسؤولة: BR1.1
ABSTRACT

Objective:

This study aimed to investigate whether interleukin-28A (IL-28A) plays a role in murine myocarditis induced by coxsackievirus B3 (CVB3), and to explore its possible mechanism involved.

Methods:

Male BALB/c mice both infected and not infected by CVB3 were randomly divided into four groups (n = 40), untreated or treated with different doses of IL-28A for 4 days, and then sacrificed on days 4 and 7 post-infection. The heart samples were collected for histopathologic examination. Cardiac viral load was determined by a plaque assay. Additionally, immunoblot analysis, TUNEL assay, and immunohistochemistry were performed to examine the expression of signal transducer, activator of transcription 1 and 2 (STAT1 and STAT2), CVB3-induced apoptosis and the expression of Bcl-2, BAX and Caspase-3.

Results:

Compared to uninfected mice, the CVB3 infected mice exhibited higher mortality rate (p < 0.001), apparent inflammation and myocardial lesion (p < 0.01), and higher cardiac viral load (p < 0.01). After CVB3 infection, IL-28A treated mice presented no death (p < 0.001), reduced inflammation and myocardial lesion (p < 0.01), and lower viral load (p < 0.01) compared to untreated mice. Besides, treatment with IL-28A markedly increased the expressions of STAT1 and STAT2, and inhibited CVB3-induced apoptosis in myocardial cells with increased ratio of Bcl-2/BAX.

Conclusion:

The antiviral and myocyte protective effects of IL-28A in CVB3-inducedmyocarditis are regulated by STAT1 and STAT2. .
الموضوعات
Key words

النص الكامل: 1 الفهرس: LILACS الموضوع الرئيسي: Antiviral Agents / Interleukins / Coxsackievirus Infections / Myocarditis المحددات: Animals اللغة: En مجلة: Braz. j. infect. dis موضوع المجلة: DOENCAS TRANSMISSIVEIS السنة: 2015 نوع: Article

النص الكامل: 1 الفهرس: LILACS الموضوع الرئيسي: Antiviral Agents / Interleukins / Coxsackievirus Infections / Myocarditis المحددات: Animals اللغة: En مجلة: Braz. j. infect. dis موضوع المجلة: DOENCAS TRANSMISSIVEIS السنة: 2015 نوع: Article