Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation
Acta cir. bras
; Acta cir. bras;30(5): 359-365, 05/2015. tab, graf
Article
ي En
| LILACS
| ID: lil-747026
المكتبة المسؤولة:
BR1.1
ABSTRACT
PURPOSE:
To evaluate a new perfusate solution to be used for ex vivo lung perfusion.METHODS:
Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative.RESULTS:
From 16 lungs blocs tested, we found no difference on weight after EVLP Steen group (SG) = 1,097±526g; Alternative Perfusion Solution (APS) = 743±248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS = 3.63 ± 1.26; SG = 2.06 ± 0.28; p = 0.009). No difference on PaO2 after EVLP (SG = 498±37.53mmHg; APS = 521±55.43mmHg, p=0.348, nor on histological analyses pulmonary injury score SG = 4.38±1.51; APS = 4.50±1.77, p=0.881; apoptotic cells count after perfusion SG = 2.4 ± 2.0 cells/mm2; APS = 4.8 ± 6.9 cells/mm2; p = 0.361).CONCLUSION:
The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution(r) on lungs from rejected brain-dead donors. .Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Organ Preservation
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Perfusion
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Lung Transplantation
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Organ Preservation Solutions
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Lung
نوع الدراسة:
Clinical_trials
المحددات:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
اللغة:
En
مجلة:
Acta cir. bras
موضوع المجلة:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
السنة:
2015
نوع:
Article
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Project document