Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification
Braz. j. microbiol
; 46(1): 279-283, 05/2015. graf
Article
ي En
| LILACS
| ID: lil-748263
المكتبة المسؤولة:
BR1.1
ABSTRACT
Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after HindIII digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes HindIII and BamHI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of BamHI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
Key words
النص الكامل:
1
الفهرس:
LILACS
الموضوع الرئيسي:
Tumor Virus Infections
/
Cattle Diseases
/
Herpesviridae Infections
نوع الدراسة:
Diagnostic_studies
/
Prognostic_studies
المحددات:
Animals
البلد/الأقليم حسب الموضوع:
America do sul
/
Brasil
اللغة:
En
مجلة:
Braz. j. microbiol
موضوع المجلة:
MICROBIOLOGIA
السنة:
2015
نوع:
Article