Enhanced Chrysene Degradation by a Mixed culture Biorem-CGBD using Response Surface Design.
Indian J Exp Biol
; 2015 May; 53(5): 256-263
Article
ي En
| IMSEAR
| ID: sea-158444
ABSTRACT
Degradation of chrysene, a four ringed highly carcinogenic polycyclic aromatic hydrocarbon (PAH) has been demonstrated by bacterial mixed culture Biorem-CGBD comprising Achromobacter xylosoxidans, Pseudomonas sp. and Sphingomonas sp., isolated from crude oil polluted saline sites at Bhavnagar coast, Gujarat, India. A full factorial Central Composite Design (CCD) using Response Surface Methodology (RSM) was applied to construct response surfaces, predicting 41.93% of maximum chrysene degradation with an experimental validation of 66.45% chrysene degradation on 15th day, using a combination of 0.175, 0.175 and 0.385 mL of OD600=1 inoculum of A. xylosoxidans, Pseudomonas sp. and Sphingomonas sp., respectively and a regression coefficient (R2) of 0.9485 indicating reproducibility of the experiment. It was observed that chrysene degradation can be successfully enhanced using RSM, making mixed culture Biorem-CGBD a potential bioremediation target for PAH contaminated saline sites.
Key words
النص الكامل:
1
الفهرس:
IMSEAR
الموضوع الرئيسي:
Polycyclic Aromatic Hydrocarbons
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Biodegradation, Environmental
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Bacteriological Techniques
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Coculture Techniques
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Chemical Phenomena
نوع الدراسة:
Prognostic_studies
اللغة:
En
مجلة:
Indian J Exp Biol
السنة:
2015
نوع:
Article