Effect of sulphur crosslinking on the stability and transition of triple helical DNA.
Indian J Biochem Biophys
; 1999 Jun; 36(3): 177-87
Article
ي En
| IMSEAR
| ID: sea-26562
ABSTRACT
In continuation to our work on order-order and order-disorder transition in triple stranded DNA when it is bounded to netropsin, we report in this communication the stabilizing/destabilizing effect of disulphide linkage on the phase dynamics of the triplex using the amended Zimm-Bragg theory. It is observed that in contrast to the sequential triplex-->duplex -->single strand melting of the uncrosslinked triplex, crosslinking causes the triplex state to melt directly to the single stranded state, with no apparent intermediary of a duplex state. Since there is no overall difference in the enthalpy of crosslinked and uncrosslinked triplexes, the transition is entropy driven.
النص الكامل:
1
الفهرس:
IMSEAR
الموضوع الرئيسي:
Thermodynamics
/
DNA
/
Molecular Sequence Data
/
Base Sequence
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Cross-Linking Reagents
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Disulfides
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Nucleic Acid Conformation
اللغة:
En
مجلة:
Indian J Biochem Biophys
السنة:
1999
نوع:
Article