Identification of human malaria parasites and detection of mixed infection in Thai patients by nested PCR.
Southeast Asian J Trop Med Public Health
; 2004 ; 35 Suppl 2(): 5-9
Article
ي En
| IMSEAR
| ID: sea-36311
ABSTRACT
The species-specific nested PCR previously described by Snounou and others, for detecting the four species of human malaria parasites, is evaluated in the current study testing 40 blood samples from malaria patients admitted during July-September, 2003, at the Hospital for Tropical Diseases, Faculty of Tropical Medicine, Mahidol University, Thailand. Parasite DNA of each blood sample was extracted and purified by QIAamp. DNA mini kit. Nested PCR was performed using genus-specific primers for the first PCR cycle and species-specific primer for the second cycle. Thin and thick smears were also made, stained with Giemsa, and examined by expert microscopists. Only one of 40 samples (2.5%) was identified as Plasmodium malariae infection by both microscopy and nested PCR. Twenty blood samples (50%) were identified as Plasmodium falciparum infections by both methods. However, 19 blood samples (47.5%) were reported as Plasmodium vivax infections by microscopic methods, whereas nested PCR could detect a mixed infection of Plasmodium vivax and Plasmodium falciparum in one sample taken from a young girl with 8 ameboid trophozoites of P. vivax per 200 white blood cells. These results demonstrated that the nested PCR assay surpasses microscopy and also offers a clear advantage in the detection of mixed infections, which is important not only for successful medical treatment, but also for the study of malaria epidemiology.
النص الكامل:
1
الفهرس:
IMSEAR
الموضوع الرئيسي:
Plasmodium
/
Species Specificity
/
Staining and Labeling
/
Thailand
/
Female
/
Humans
/
Male
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Adolescent
نوع الدراسة:
Diagnostic_studies
/
Evaluation_studies
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
En
مجلة:
Southeast Asian J Trop Med Public Health
السنة:
2004
نوع:
Article