Myristica fragrans Houtt. methanolic extract induces apoptosis in a human leukemia cell line through SIRT1 mRNA downregulation.
Article
ي En
| IMSEAR
| ID: sea-42375
ABSTRACT
BACKGROUND:
Myristica fragrans Houtt. (nutmeg) contains antibacterial, antiviral and anti-cancer activities. However the mechanisms underlying those activities have not been clearly explained.OBJECTIVE:
To study the effect of Myristica fragrans Houtt. methanolic extract on Jurkat human leukemia T cell line. MATERIAL ANDMETHOD:
Methanol extract of Myristica fragrans Houtt. (Myristicaceae) was used to study the effect on Jurkat cell metabolic activity using an MTT assay and on apoptosis using annexin V staining. Expression of SIRT1 gene was determined by RT-PCR.RESULTS:
At the concentrations 50 and 100 ig/mL, the methanol extract of Myristica fragrans Houtt significantly inhibited Jurkat cell proliferation and induced apoptosis as detected by annexin V staining. Downregulation of SIRT1 mRNA expression in Jurkat cells was observed even when the amount of methanol extract was 10 microg/mL.CONCLUSION:
Methanol extract of Myristica fragrans Houtt induced apoptosis of Jurkat leukemia T cell line in a mechanisms involving SIRTI mRNA downregulation.
النص الكامل:
1
الفهرس:
IMSEAR
الموضوع الرئيسي:
Humans
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RNA, Messenger
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Plant Extracts
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Down-Regulation
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Gene Expression
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Pilot Projects
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Spices
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Apoptosis
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Annexin A5
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Myristica
اللغة:
En
السنة:
2007
نوع:
Article