L-asparaginase activity in Aeromonas sp. isolated from freshwater mussel.
Indian J Exp Biol
; 2000 Nov; 38(11): 1143-6
Article
ي En
| IMSEAR
| ID: sea-57153
ABSTRACT
Aeromonas sp. from Lamellidens marginalis produced L-asparaginase when grown at 37 degrees C. The optimum enzyme activity was at pH 9 when temperature was 45 degrees C. Half-life of partially purified enzyme at 50 degrees C and 55 degrees C was 35 and 20 min, respectively. Activation and deactivation energies of partially purified enzyme were 17.48 and 24.86 kcal mol-1 respectively. The enzyme exhibited a Km (L-asparagine) value of 4.9 x 10(-6) mol l-1 and a Vmax of 9.803 IU ml-1. Three metal ions inhibited the enzyme activity at 10-20 mumol l-1 concentrations. Catalytic activity was also inhibited by EDTA, iodoacetic acid, parachloromercuribenzoic acid and phenylmethylsulphonyl fluoride at 0.1 mumol l-1.
النص الكامل:
1
الفهرس:
IMSEAR
الموضوع الرئيسي:
Asparaginase
/
Bivalvia
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Aeromonas
/
Hot Temperature
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Hydrogen-Ion Concentration
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Animals
/
Metals
اللغة:
En
مجلة:
Indian J Exp Biol
السنة:
2000
نوع:
Article