Use of filter paper disks for hepatitis A surveillance.
Article
ي En
| IMSEAR
| ID: sea-65415
ABSTRACT
BACKGROUND:
Venous blood collection is a cumbersome and uncomfortable procedure during hepatitis A surveillance. Collection of capillary blood by finger prick is an alternative method.AIM:
To evaluate the reactivity of capillary blood/anti-hepatitis A virus (HAV) IgG stored on filter paper disks for detection of anti-HAV antibody.METHODS:
Venous blood specimens were collected from healthy individuals. Simultaneous capillary blood specimens obtained by finger prick were stored on filter paper disks. A reference standard of anti-HAV IgG in known concentrations was spotted on filter paper disks. The reactivities of anti-HAV IgG and capillary blood specimens eluted from filter paper disks were tested by blocking ELISA for detection of anti-HAV antibody. The results were evaluated by comparing optical density (OD) and neutralization values with those obtained for WHO anti-HAV IgG stored in liquid phase and homologous venous blood specimens, respectively.RESULTS:
Among both venous and capillary-blood specimens stored for 10 days, percent neutralization shown by the same 46 specimens was > 50 and that of the same 3 specimens was < 50, indicating anti-HAV positivity and negativity, respectively. There was significant correlation between the OD values displayed by anti-HAV IgG from liquid phase and that eluted from filter paper disk (p < 0.01). Sixteen serum specimens stored for a period of 2 months showed results similar to those of the corresponding filter paper disk elutes.CONCLUSION:
Use of filter paper disks could be a suitable choice for pre- and post-immunization collection of blood specimens during hepatitis A surveillance.
النص الكامل:
1
الفهرس:
IMSEAR
الموضوع الرئيسي:
Paper
/
Reference Values
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Specimen Handling
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Female
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Humans
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Male
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Hepatitis Antibodies
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Population Surveillance
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Bacteriological Techniques
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Sensitivity and Specificity
نوع الدراسة:
Clinical_trials
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Diagnostic_studies
/
Screening_studies
اللغة:
En
السنة:
2000
نوع:
Article