Identification of metabolites in rat plasma,bile,urine and feces after oral administration of Cinnamomi Cortex aqueous extract by UPLC-Qtrap-MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4720-4727, 2019.
Article
ي Zh
| WPRIM
| ID: wpr-1008251
المكتبة المسؤولة:
WPRO
ABSTRACT
An ultra-performance liquid chromatography hybrid triple quadrupole-linear ion trap mass spectrometry(UPLC-QtrapMS) method was established to identify the metabolites in rat plasma,bile,urine and feces after oral administration of Cinnamomi Cortex(CC) aqueous extract. Several survey experiments,such as enhanced mass spectrum scan(EMS),precursor ion scan(PI),neutral loss scan(NL) and multiple ions monitoring(MIM) were applied to search target components,and two separate enhanced product ion(EPI) scans were triggered via information-dependent acquisition(IDA) method to generate the MS/MS spectra. According to the mass spectrometric data collected from reference standards and reported literature,the structures of metabolites were deduced. A total of76 metabolites and 5 original compounds were tentatively identified in rats after oral administration of CC aqueous extract. Deglycosylation,methylation,sulfonation,and glucuronidation were observed as the primary metabolic pathways for the chemical constituents of CC. These data are able to benefit the clarification of the therapeutic material basis,the clinical usage and further R&D of CC.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Bile
/
Drugs, Chinese Herbal
/
Administration, Oral
/
Chromatography, High Pressure Liquid
/
Cinnamomum zeylanicum
/
Tandem Mass Spectrometry
/
Feces
المحددات:
Animals
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2019
نوع:
Article