Application of MALDI-mass spectrometry imaging in spatial distribution of secondary metabolites in medicinal plants -- a case study of Lepdium meyenii root / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 596-601, 2020.
Article
ي Zh
| WPRIM
| ID: wpr-1008543
المكتبة المسؤولة:
WPRO
ABSTRACT
Matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) is a novel technique for in-situ distribution of various substances in tissue without labeling. This technique is increasingly applied to the study of medicinal plants owing to its high spatial resolution and its potential of in-situ analysis in small molecules. In this study, the structural information and their fragmentation patterns of the midazole alkaloids(1,3-dibenzyl-4,5-dimethylimidazolium chloride and 1,3-dibenzyl-2,4,5-trimethylimi-dazolium chloride) and benzylglucosinolate in the medicinal plant Maca(Lepdium meyeni) root were analyzed by ultra-high-performance liquid phase combined with LTQ-Orbitrap mass spectrometry(UHPLC-HR-MS). The localization of these active ingredients in the cross-sections of Maca root was performed by MALDI-MSI. These results demonstrated that the two types of imidazole alkaloids had a similar distributed pattern. They were located more in the cortex and the periderm than those in the medulla of a lateral root, while the localization of benzylglucosinolate was concentrated in the center of the root rather than in the cortex and the periderm. The precise spatial distribution of various secondary metabolites in tissue provides an important scientific basis for the accumulation of medicinal plant active ingredients in tissues. In addition, this imaging method is a promising technique for the rapid evaluation and identification of the active ingredients of traditional Chinese medicine in plant tissues, as well as assisting the research on the processing of medicinal plants.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Plants, Medicinal
/
Chromatography, High Pressure Liquid
/
Plant Roots
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Lepidium
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Phytochemicals
/
Secondary Metabolism
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2020
نوع:
Article