Accuracy of Polymerase Chain Reaction Based Assays for Trichomonas Vaginitis:A Meta-Analysis / 现代检验医学杂志

ABSTRACT

Objective To investigate the accuracy of polymerase chain reaction(PCR)based assays for Trichomonas Vaginitis.Methods The research literature on the detection of TV based on PCR method since the establishment of the database to May 30,2023 was retrieved from China National Knowledge Infrastructure(CNKI),Wanfang Data Knowledge Service Platform,VIP Database and PubMed Database.After literature screening and data extraction,Meta-analysis was performed using software RevMan 5.4.1 and web service Meta-Disc 2.0.Results A total of 36 literatures and 16 454 clinical samples were included.The results of meta-analysis showed that the combined sensitivity and specificity of polymerase chain reaction for the detection of TV were 0.972(0.943,0.987)and 0.979(0.968,0.986),respectively,the diagnostic odds ratio was 1 643.398(673.168,4 012.008),the positive likelihood ratio was 46.209(30.549,69.897),and the negative likelihood ratio was 0.028(0.013,0.059).Subgroup analysis showed that clinical samples of different genders would not affect the accuracy of polymerase chain reaction,but the accuracy of different PCR detection methods was different.Conclusion The accuracy of polymerase chain reaction in the detection of TV was high and it has good application value.