Clathrin and Lipid Raft-dependent Internalization of Porphyromonas gingivalis in Endothelial Cells
International Journal of Oral Biology
; : 131-136, 2014.
Article
ي En
| WPRIM
| ID: wpr-102299
المكتبة المسؤولة:
WPRO
ABSTRACT
Porphyromonas gingivalis is one of the most important periodontal pathogens and has been to known to invade various types of cells, including endothelial cells. The present study investigated the mechanisms involved in the internalization of P. gingivalis in human umbilical vein endothelial cells (HUVEC). P. gingivalis internalization was reduced by clathrin and lipid raft inhibitors, as well as a siRNA knockdown of caveolin-1, a principal molecule of lipid raft-related caveolae. The internalization was also reduced by perturbation of actin rearrangement, while microtubule polymerization was not required. Furthermore, we found that Src kinases are critical for the internalization of P. gingivalis into HUVEC, while neither Rho family GTPases nor phosphatidylinositol 3-kinase are required. Taken together, this study indicated that P. gingivalis internalization into endothelial cells involves clathrin and lipid rafts and requires actin rearrangement associated with Src kinase activation.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Phosphotransferases
/
Polymers
/
Actins
/
Clathrin
/
Porphyromonas gingivalis
/
Src-Family Kinases
/
Caveolae
/
RNA, Small Interfering
/
Endothelial Cells
/
Caveolin 1
المحددات:
Humans
اللغة:
En
مجلة:
International Journal of Oral Biology
السنة:
2014
نوع:
Article