Applications of CRISPR/Cas-based biosensing strategies in rapid pathogen detection / 中华检验医学杂志

ABSTRACT

In recent years, infectious pathogens have received sustained attention because of their serious impact on the world′s health and socio-economic infrastructure. The existing common detection methods lack a certain sensitivity and specificity, the process is tedious, and they rely on more expensive auxiliary instruments and equipment. On the other hand, clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats associated protein (CRISPR/Cas) has been widely used in the monitoring of infectious diseases due to its high specificity, sensitivity, and rapid programmable characteristics, and has shown important potential significance in the research of new biosensors in nucleic acid detection. This paper describes the functional mechanism of Cas protein commonly used in CRISPR/Cas system, summarizes the latest research progress of new biosensor technology based on CRISPR/Cas system in the field of infectious disease detection, and looks forward to the technical problems to be solved and the future development direction. With more research advancement, more types of biosensing platforms based on CRISPR/Cas system are expected to be developed, paving the way for the application of POCT in the field of rapid pathogen detection.