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Effect of ultra short-term low-dose irradiation on invasive properties of glioma cells: an in vitro study / 中华神经医学杂志
Chinese Journal of Neuromedicine ; (12): 881-884, 2014.
Article ي Zh | WPRIM | ID: wpr-1034025
المكتبة المسؤولة: WPRO
ABSTRACT
Objective To evaluate the effect of ultra short-term low-dose gamma knife radiation on invasive properties of glioma cells at the genetic level.Methods Malignant glioma cell lines U87 and U251 were treated with gamma knife radiation,respectively,with a marginal doses of 0,4,6,8 and 10 Gy (all to the 80% isodose line).Real time-PCR was used to measure the mRNA expressions of AKT-1,AKT-2,β-catenin and TCF-4 at 30 min after radiation.Results The expressions of AKT-1,AKT-2,β-catenin,TCF-4 in glioma cell lines after low-dose gamma knife radiation with a marginal dose of 6-8 Gy were significantly increased as compared with those after 0 Gy (P<0.05); reduced expressions ofAKT-1,AKT-2,β-catenin and TCF-4 after a marginal dose of 10 Gy were found as compared with those after 0 Gy (P<0.05); the β-catenin and AKT-1 mRNA expressions in U87 cells and TCF-4,AKT-1 and AKT-2 mRNA expressions in U251 cells enjoyed the most obvious increase at a marginal dose of 6 Gy; the TCF-4 and AKT-2 mRNA expressions in U87 cells and β-catenin mRNA expression in U251 cells enjoyed the most obvious increase at a marginal dose of 8 Gy.Conclusion Low-dose gamma knife radiation (6-8 Gy) could increase the β-catenin,TCF-4,AKT-1 and AKT-2 expressions,while high dose (10 Gy) could inhibit these effects in a short time period of 30 min.
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النص الكامل: 1 الفهرس: WPRIM اللغة: Zh مجلة: Chinese Journal of Neuromedicine السنة: 2014 نوع: Article
النص الكامل: 1 الفهرس: WPRIM اللغة: Zh مجلة: Chinese Journal of Neuromedicine السنة: 2014 نوع: Article