Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
; : 1602-1608, 2001.
Article
ي Ko
| WPRIM
| ID: wpr-111971
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE:
To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes.METHODS:
After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated.RESULTS:
The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group.CONCLUSION:
The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial.Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Oocytes
/
Tubulin
/
Cryopreservation
/
Dimethyl Sulfoxide
/
Incidence
/
Freezing
/
Spindle Apparatus
نوع الدراسة:
Incidence_studies
/
Prognostic_studies
المحددات:
Animals
اللغة:
Ko
مجلة:
Korean Journal of Obstetrics and Gynecology
السنة:
2001
نوع:
Article