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Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes / 대한산부인과학회잡지
Article ي Ko | WPRIM | ID: wpr-111971
المكتبة المسؤولة: WPRO
ABSTRACT

OBJECTIVE:

To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes.

METHODS:

After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated.

RESULTS:

The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group.

CONCLUSION:

The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial.
الموضوعات
Key words
النص الكامل: 1 الفهرس: WPRIM الموضوع الرئيسي: Oocytes / Tubulin / Cryopreservation / Dimethyl Sulfoxide / Incidence / Freezing / Spindle Apparatus نوع الدراسة: Incidence_studies / Prognostic_studies المحددات: Animals اللغة: Ko مجلة: Korean Journal of Obstetrics and Gynecology السنة: 2001 نوع: Article
النص الكامل: 1 الفهرس: WPRIM الموضوع الرئيسي: Oocytes / Tubulin / Cryopreservation / Dimethyl Sulfoxide / Incidence / Freezing / Spindle Apparatus نوع الدراسة: Incidence_studies / Prognostic_studies المحددات: Animals اللغة: Ko مجلة: Korean Journal of Obstetrics and Gynecology السنة: 2001 نوع: Article