Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection / 대한수의학회지
Korean Journal of Veterinary Research
; : 95-101, 2013.
Article
ي Ko
| WPRIM
| ID: wpr-119240
المكتبة المسؤولة:
WPRO
ABSTRACT
The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Salmonella
/
Salmonella typhimurium
/
Sprains and Strains
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Immunity, Mucosal
/
Salmonella enterica
/
Immunity, Humoral
/
Antibodies
المحددات:
Animals
/
Female
/
Humans
اللغة:
Ko
مجلة:
Korean Journal of Veterinary Research
السنة:
2013
نوع:
Article