Effects of DA-9701, a Novel Prokinetic Agent, on Phosphorylated Extracellular Signal-Regulated Kinase Expression in the Dorsal Root Ganglion and Spinal Cord Induced by Colorectal Distension in Rats
Gut and Liver
; : 140-147, 2014.
Article
ي En
| WPRIM
| ID: wpr-123199
المكتبة المسؤولة:
WPRO
ABSTRACT
BACKGROUND/AIMS: DA-9701, a standardized extract of Pharbitis Semen and Corydalis Tuber, is a new prokinetic agent that exhibits an analgesic effect on the abdomen. We investigated whether DA-9701 affects visceral pain induced by colorectal distension (CRD) in rats. METHODS: A total of 21 rats were divided into three groups: group A (no CRD+no drug), group B (CRD+no drug), and group C (CRD+DA-9701). Expression of pain-related factors, substance P (SP), c-fos, and phosphorylated extracellular signal-regulated kinase (p-ERK) in the dorsal root ganglion (DRG) and spinal cord was determined by immunohistochemical staining and Western blotting. RESULTS: The proportions of neurons in the DRG and spinal cord expressing SP, c-fos, and p-ERK were higher in group B than in group A. In the group C, the proportion of neurons in the DRG and spinal cord expressing p-ERK was lower than that in group B. Western blot results for p-ERK in the spinal cord indicated a higher level of expression in group B than in group A and a lower level of expression in group C than in group B. CONCLUSIONS: DA-9701 may decrease visceral pain via the downregulation of p-ERK in the DRG and spinal cord.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Rectum
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Spinal Cord
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Substance P
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Down-Regulation
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Proto-Oncogene Proteins c-fos
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Rats, Sprague-Dawley
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Colon
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Plant Preparations
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Extracellular Signal-Regulated MAP Kinases
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Dilatation, Pathologic
المحددات:
Animals
اللغة:
En
مجلة:
Gut and Liver
السنة:
2014
نوع:
Article