Induction of proliferation in resting B-cells by a factor released by activated mouse spleen cells
Experimental & Molecular Medicine
; : 199-204, 1998.
Article
ي En
| WPRIM
| ID: wpr-159769
المكتبة المسؤولة:
WPRO
ABSTRACT
Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Polymyxin B
/
Protein Denaturation
/
Spleen
/
Thymus Gland
/
Bone Marrow
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B-Lymphocytes
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Chymotrypsin
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Trypsin
/
Cell Division
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Chromatography, High Pressure Liquid
المحددات:
Animals
اللغة:
En
مجلة:
Experimental & Molecular Medicine
السنة:
1998
نوع:
Article