Isolation of Subgroup J Avian Leukosis Virus in Korea
Journal of Veterinary Science
; : 71-74, 2002.
Article
ي En
| WPRIM
| ID: wpr-172833
المكتبة المسؤولة:
WPRO
ABSTRACT
Two subgroup J avian leukosis viurses (ALVs) were isolated from broiler breeder flocks, in which myeloid leukosis had occurred. The isolates could be classified as subgroup J ALV. by the positive reaction in polymerase chain reaction (PCR) with primers specific for subgroup J ALV. Two isolates replicated in chicken embryo fibroblast (CEF) cells from the alv6 chicken line in which cells are resistant to subgroup A and E ALVs. In in vitro serum neutralization tests with other subgroup ALVs including ADOL-Hc1, the prototype of subgroup J ALVs isolated in the United States of America, two isolates were partially neutralized by antibody to ADOL-Hc1, indicating that Korean isolates and ADOL-Hc1 may be antigenically related, but not identical. When the PCR was done with a primer pair designed to amplify genes of E element and long terminal repeat of proviral DNA, the PCR product size of one isolate (KOAL-PET) was smaller than that of ADOL-Hc1, suggesting that some sequences in these regions are deleted.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Poultry Diseases
/
Avian Leukosis
/
Neutralization Tests
/
Cell Line
/
Chickens
/
Polymerase Chain Reaction
/
Avian Leukosis Virus
/
Korea
/
Antibodies, Viral
/
Antigens, Viral
المحددات:
Animals
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
En
مجلة:
Journal of Veterinary Science
السنة:
2002
نوع:
Article