Real-Time PCR analysis of af4 and dek genes expression in acute promyelocytic leukemiat (15;17)patients
Experimental & Molecular Medicine
; : 279-282, 2004.
Article
ي En
| WPRIM
| ID: wpr-173474
المكتبة المسؤولة:
WPRO
ABSTRACT
Among several newly identified oncogenes, dek and af4 are attractive targets for researchers interested with leukemia. In this study quantitative Real-Time RT-PCR technique was used to define alterations in expression of dek and af4 genes associated with acute promyelocytic leukaemia (APL) t (15; 17). RNA samples obtained from bone marrow aspirates of fourteen APL patients, cDNA portions were labelled with Syber Green 1 dye and LightCycler analysis have been performed. Expression changes in patients were found not significant in comparison to healthy donors for af4 (P=0.192) and dek (P= 0.0895). We suggest that af4 gene may have a role in leukomogenesis restricted to lymphoblastic lineage; also further studies must carry on with a larger series of patients in order to understand the relationship between the dek gene and APL. Our study was the first attempt for analysing dek and af4 genes in APL t (15; 17) patients by quantitative Real-Time RT-PCR. This rapid and sensitive method could be used to screen these genes in different types of leukaemia.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Translocation, Genetic
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Chromosomes, Human, Pair 15
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Chromosomes, Human, Pair 17
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RNA, Messenger
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Nuclear Proteins
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Chromosomal Proteins, Non-Histone
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Leukemia, Promyelocytic, Acute
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Down-Regulation
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Gene Expression
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Up-Regulation
المحددات:
Adolescent
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Adult
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Child
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Child, preschool
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Female
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Humans
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Male
اللغة:
En
مجلة:
Experimental & Molecular Medicine
السنة:
2004
نوع:
Article