Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy / 대한의료정보학회지
Healthcare Informatics Research
; : 238-242, 2016.
Article
ي En
| WPRIM
| ID: wpr-177091
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVES: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining. METHODS: We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods. RESULTS: An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging. CONCLUSIONS: The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Staining and Labeling
/
Image Processing, Computer-Assisted
/
Eosine Yellowish-(YS)
/
Microscopy, Confocal
/
Diagnosis
/
Fluorescence
/
Hematoxylin
/
Methods
/
Microscopy
نوع الدراسة:
Diagnostic_studies
اللغة:
En
مجلة:
Healthcare Informatics Research
السنة:
2016
نوع:
Article