Effects of interleukin-10 on chemokine KC gene expression by mouse peritoneal macrophages in response to Candida albicans
Journal of Korean Medical Science
; : 480-486, 1999.
Article
ي En
| WPRIM
| ID: wpr-187372
المكتبة المسؤولة:
WPRO
ABSTRACT
Chemokine KC has been considered to be a murine homologue of human GRO/MGSA and was identified as chemoattractant for monocytes and neutrophils. This study examined the expression of KC mRNA in thioglycollate-elicited mouse peritoneal macrophages that were stimulated in vitro with Candida albicans (CA). Also examined were the inhibitory effects of IL-10 on the CA-induced expression of KC gene by Northern blot analysis. CA was found to induce chemokine gene expression in a gene-specific manner, CXC chemokine IP-10 mRNA expression was not detected in CA-stimulated macrophages. Maximum KC mRNA expression was observed approximately 2 hr after adding CA. The inhibitory action of IL-10 to CA-induced KC mRNA expression on mouse peritoneal macrophages was independent on concentration and stimulation time of IL-10 and was observed approximately one hour after adding IL-10 and CA simultaneously. IL-10 produced a decrease in the stability of KC mRNA, and CA-stimulated macrophages with cycloheximide blocked the suppressive effect of IL-10. These results suggest that CA also induces chemokine KC from macrophages, and IL-10 acts to destabilize CA-induced KC mRNA and de novo synthesis of an intermediate protein is a part of the IL-10 suppressive mechanism.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Candida albicans
/
RNA, Messenger
/
Cells, Cultured
/
Chemotactic Factors
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Gene Expression Regulation
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Blotting, Northern
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Growth Substances
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Interleukin-10
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Nucleic Acid Synthesis Inhibitors
/
Dactinomycin
نوع الدراسة:
Prognostic_studies
المحددات:
Animals
اللغة:
En
مجلة:
Journal of Korean Medical Science
السنة:
1999
نوع:
Article