Trichomonas vaginalis α-Actinin 2 Modulates Host Immune Responses by Inducing Tolerogenic Dendritic Cells via IL-10 Production from Regulatory T Cells
The Korean Journal of Parasitology
; : 375-384, 2017.
Article
ي En
| WPRIM
| ID: wpr-203201
المكتبة المسؤولة:
WPRO
ABSTRACT
Trichomonas vaginalis is a pathogen that triggers severe immune responses in hosts. T. vaginalis α-actinin 2, Tvα-actinin 2, has been used to diagnose trichomoniasis. This study was undertaken to examine the role of Tvα-actinin 2 as an antigenic molecule to induce immune responses from humans. Western blot analysis using anti-Tvα-actinin 2 antibodies indicated its presence in the secreted proteins of T. vaginalis. ELISA was employed to measure cytokine production by vaginal epithelial cells, prostate cells, mouse dendritic cells (DCs), or T cells stimulated with T. vaginalis or Tvα-actinin 2 protein. Both T. vaginalis and rTvα-actinin 2 induced cytokine production from epithelial cell lines, including IL-10. Moreover, CD4+CD25− regulatory T cells (Treg cells) incubated with rTvα-actinin 2-treated DCs produced high levels of IL-10. These data indicate that Tvα-actinin 2 modulates immune responses via IL-10 production by Treg cells.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Prostate
/
Trichomonas
/
Trichomonas vaginalis
/
Dendritic Cells
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Enzyme-Linked Immunosorbent Assay
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T-Lymphocytes
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Blotting, Western
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Interleukin-10
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T-Lymphocytes, Regulatory
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Epithelial Cells
المحددات:
Animals
/
Humans
اللغة:
En
مجلة:
The Korean Journal of Parasitology
السنة:
2017
نوع:
Article