Effect of the Physiologically Active Compounds in Phlomidis Radix on Cell Cycle Regulation in Human Gingival Fibroblasts / 대한치주과학회지

ABSTRACT

The purpose of present study was to investigate the effects of physiologically active compound (SD62-122) from Phlomidis Radix on the cell cycle progression and its molecular mechanism in human gingival fibroblasts(HGFs). For this purpose, fibroblasts were isolated and cultured from excisioned gingiva during crown lengthening procedure in healthy adult. The following parameter were evaluated that there are cell number counting, MTT assay, cell cycle progression, western blot analysis. The cell number and MTT assay of primary cultured fibroblast was not increased at 2 days but significant increased compare to negative control at 3days(p<0.05). S phase was increased and G1 phase decreased in both 10(-8)M and 10(-9)M of SD62-122 in cell cycle analysis. The cell cycle regulation protein levels of Cyclin D1, Cyclin E, cdk 2, cdk 4 and cdk 6 were increased compare to control in both 10(-8)M and 10(-9)M of SD62-122. The protein levels of p21 and p53 were decreased compare to control, but the level of pRb was not changed compare to control in 10(-9)M of SD2-122. These results suggested that physiologically active compound (SD62-122) isolated from Phlomidis Radix increases the cell proliferation and cell cycle progression in HGFs, which is linked to increased cell cycle regulation protein levels of Cyclin D1, Cyclin E, cdk 2, cdk 4 and cdk 6, and decreased the levels of p21, p53.