Development of a Synthetic Surfactant Using a Surfactant Protein-C Peptide Analog: In Vitro Studies of Surface Physical Properties
Yonsei Medical Journal
; : 203-208, 2016.
Article
ي En
| WPRIM
| ID: wpr-220781
المكتبة المسؤولة:
WPRO
ABSTRACT
PURPOSE: Pulmonary surfactant (PS) replacement has been the gold standard therapy for neonatal respiratory distress syndrome; however, almost all commercial PSs contain animal proteins. We prepared a synthetic PS by using a human surfactant protein (SP) analog and evaluated its in vitro properties. MATERIALS AND METHODS: A peptide sequence (CPVHLKRLLLLLLLLLLLLLLLL) of human SP-C was chosen to develop the peptide analog (SPa-C). The new synthetic SP-C PS (sSP-C PS) was synthesized from SPa-C, dipalmitoyl phosphatidylcholine, phosphatidyl glycerol, and palmitic acid. Physical properties of the sSP-C PS were evaluated by measuring the maximum and minimum surface tensions (STs), surfactant spreading, and adsorption rate. In addition, we recorded an ST-area diagram. The data obtained on sSP-C PS were subsequently compared with those of purified natural bovine surfactant (PNBS), and the commercial product, Surfacten(R). RESULTS: The sSP-C PS and Surfacten(R) were found to have maximum ST values of 32-33 mN/m, whereas that of PNBS was much lower at 19 mN/m. The minimum ST values of all three products were less than 10 mN/m. The values that were measured for the equilibrium ST of rapidly spreading sSP-C PS, Surfacten(R), and PNBS were 27, 27, and 24 mN/m, respectively. The surface adsorptions were found to be the same for all three PSs (20 mN/m). ST-area diagrams of sSP-C PS and Surfacten(R) revealed similar properties. CONCLUSION: In an in vitro experiment, the physical properties exhibited by sSP-C PS were similar to those of Surfacten(R). Further study is required to evaluate the in vivo efficacy.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Respiratory Distress Syndrome, Newborn
/
Surface-Active Agents
/
Surface Properties
/
Surface Tension
/
1,2-Dipalmitoylphosphatidylcholine
/
Pulmonary Surfactants
/
C-Peptide
/
Amino Acid Sequence
/
Adsorption
/
Pulmonary Surfactant-Associated Protein C
المحددات:
Animals
/
Humans
اللغة:
En
مجلة:
Yonsei Medical Journal
السنة:
2016
نوع:
Article