Protective effects of remifentanil against H₂O₂-induced oxidative stress in human osteoblasts
Journal of Dental Anesthesia and Pain Medicine
; : 263-271, 2016.
Article
ي En
| WPRIM
| ID: wpr-222966
المكتبة المسؤولة:
WPRO
ABSTRACT
BACKGROUND: Bone injury is common in many clinical situations, such as surgery or trauma. During surgery, excessive reactive oxygen species (ROS) production decreases the quality and quantity of osteoblasts. Remifentanil decreases ROS production, reducing oxidative stress and the inflammatory response. We investigated remifentanil's protective effects against H₂O₂-induced oxidative stress in osteoblasts. METHODS: To investigate the effect of remifentanil on human fetal osteoblast (hFOB) cells, the cells were incubated with 1 ng/ml of remifentanil for 2 h before exposure to H2O2. For induction of oxidative stress, hFOB cells were then treated with 200 µM H₂O₂ for 2 h. To evaluate the effect on autophagy, a separate group of cells were incubated with 1 mM 3-methyladenine (3-MA) before treatment with remifentanil and H₂O₂. Cell viability and apoptotic cell death were determined via MTT assay and Hoechst staining, respectively. Mineralized matrix formation was visualized using alizarin red S staining. Western blot analysis was used to determine the expression levels of bone-related genes. RESULTS: Cell viability and mineralized matrix formation increased on remifentanil pretreatment before exposure to H₂O₂-induced oxidative stress. As determined via western blot analysis, remifentanil pretreatment increased the expression of bone-related genes (Col I, BMP-2, osterix, and TGF-β). However , pretreatment with 3-MA before exposure to remifentanil and H₂O₂ inhibited remifentanil's protective effects on hFOB cells during oxidative stress. CONCLUSIONS: We showed that remifentanil prevents oxidative damage in hFOB cells via a mechanism that may be highly related to autophagy. Further clinical studies are required to investigate its potential as a therapeutic agent.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Osteoblasts
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Autophagy
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Cell Survival
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Blotting, Western
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Cell Death
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Reactive Oxygen Species
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Oxidative Stress
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Miners
المحددات:
Humans
اللغة:
En
مجلة:
Journal of Dental Anesthesia and Pain Medicine
السنة:
2016
نوع:
Article