The Cell Cycle Regulatory Effects of High Dose 5-fluorouracil on Breast Cancer Cell Line
Immune Network
; : 60-64, 2002.
Article
ي Ko
| WPRIM
| ID: wpr-228524
المكتبة المسؤولة:
WPRO
ABSTRACT
BACKGROUND:
Chemotherapy with 5-fluorouracil (5-FU) has been one of the mainstay in breast cancer treatment. The effects of high dose 5-FU on cell cycle regulation were studied in breast caner cells.METHODS:
A breast cancer cell line MCF-7 was used. Protein expressions of G1/S cyclins, p21(Waf1/Cip1), cdk2, E2F1 and retinoblastoma were tested by western blot analysis. Immunoprecipitation and immune complex kinase assay were done for the assessment of E2F1/RB interacton and the activity of cdk2 respectively.RESULTS:
p21(Waf1/Cip1) expression was barely detectable in control cells. With addition of 5-FU level of p21(Waf1/Cip1) were induced and cyclin D3 level was decreased as cell growth decreases. In accordance with increased expression of p21(Waf1/Cip1), cyclin E-associated cdk2 kinase activity was reduced. Retinoblastoma protein (RB) became dephosphorylated and E2F-1 binding activity with RB was increased.CONCLUSION:
In this situation of high concentration of 5-FU breast cancer cells tend to be G1/S cell cycle arrested. Overexpression of p21(Waf1/Cip1) and dephosphorylation of RB may mediate the effectss of 5-FU by inhibiting E2F-1 activity, which contributes to G1/S cell cycle arrest. These results could be an indicating landmark for further study of high dose chemotherapy with 5-FU.Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Phosphotransferases
/
Retinoblastoma
/
Breast
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Breast Neoplasms
/
Cell Cycle
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Cell Line
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Blotting, Western
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Retinoblastoma Protein
/
Cyclins
/
Immunoprecipitation
اللغة:
Ko
مجلة:
Immune Network
السنة:
2002
نوع:
Article