Determination of benzopyrene in red clover extract by high performance liquid chromatography with spectrofluorescence detector / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract.</p><p><b>METHOD</b>Analysis was performed on Hypersil C18 column (4. 6 mm x 200 mm, 5 microm) with acetonitrile and water (70: 30) as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>The linear range was 0.93-37.2 ng x mL(-1), and r = 0.9992 (n = 5). The average recovery of benzopyrene was 85.2% (n = 9).</p><p><b>CONCLUSION</b>The method was convenient and accurate with good recovery.</p>