The expression of VP19 gene from prawn white spot syndrome virus in silkworm, Bombyx mori using host range-expanded HyNPV / 生物工程学报
Chinese Journal of Biotechnology
; (12): 837-839, 2005.
Article
ي Zh
| WPRIM
| ID: wpr-237064
المكتبة المسؤولة:
WPRO
ABSTRACT
Prawn white spot syndrome is caused by the pathogen prawn white spot syndrome virus (WSSV). VP19 is a vesicle membrane protein of WSSV. HyNPV (Hybrid of AcNPV and BmNPV) constructed by the recombination of BmNPV and AcNPV is a new hybrid virus having both of their advantages. The recombinant transfer vector pBlueBicHisC-vp19 and recombinant baculovirus HyNPV-VP19 were constructed on the basis of the successful cloning of VP19. Newly-molted silkworms Bombyx mori of fifth instar were inoculated by the recombinant virus. SDS-PAGE and Western blotting analysis showed a specific band, about 21kD, which was consistent with the expectation suggesting that the WSSV-VP19 gene was successfully expressed in silkworm bodies.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Bombyx
/
Virology
/
Virus Replication
/
Recombinant Fusion Proteins
/
Viral Envelope Proteins
/
Baculoviridae
/
Penaeidae
/
White spot syndrome virus 1
/
Genetic Vectors
/
Genetics
المحددات:
Animals
اللغة:
Zh
مجلة:
Chinese Journal of Biotechnology
السنة:
2005
نوع:
Article