Over-expression of hypoxia-inducible factor-1alpha induces epithelial-mesenchymal transition in LNCaP cells in vitro and in vivo / 中华男科学杂志
National Journal of Andrology
; (12): 23-28, 2012.
Article
ي Zh
| WPRIM
| ID: wpr-239014
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of the over-expression of hypoxia-inducible factor-1alpha (HIF-1alpha) on the epithelial-mesenchymal transition (EMT) and invasive potency of LNCaP cells in vitro and in vivo.</p><p><b>METHODS</b>We cultured LNCaP cells stably expressing HIF-1alpha (LNCaP/HIF-1alpha) and LNCaP cells, identified the over-expression of HIF-1alpha, determined the proliferation of the two cell lines by MTT assay and the level of PSA in the supernatant of culture medium, and detected the anchorage independent growth by soft-agar colony formation assay. A subcutaneous tumor model was established in nude mice by injecting LNCaP/HIF-1alpha and LNCaP cells followed by observation of the tumor growth. Tumor specimens were obtained for immunohistochemistry.</p><p><b>RESULTS</b>The over-expression of HIF-1alpha was confirmed in the LNCaP/HIF-1alpha cells by immunofluorescence staining and Western blotting. The level of PSA was obviously decreased in LNCaP/HIF-1alpha as compared with that in LNCaP cells. MTT assay identified the increased proliferation of LNCaP/HIF-1alpha cells. The cell colony forming ability of the LNCaP cells was significantly lower than that of the LNCaP/HIF-1alpha cells. The rate of tumorigenesis was increased and its time shortened in the LNCaP/HIF-1alpha group. Immunohistochemistry revealed an up-regulated expression of vimentin and a down-regulated expression of E-cadherin in the tumor specimens.</p><p><b>CONCLUSION</b>The overexpression of HIF-1alpha can up-regulate the expression of vimentin and down-regulate the expression of E-cadherin, which may enhance the invasive potency of LNCaP cells by inducing EMT.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
/
Prostatic Neoplasms
/
Cell Hypoxia
/
Cell Line, Tumor
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Epithelial-Mesenchymal Transition
/
Metabolism
/
Mice, Nude
نوع الدراسة:
Prognostic_studies
المحددات:
Animals
/
Humans
/
Male
اللغة:
Zh
مجلة:
National Journal of Andrology
السنة:
2012
نوع:
Article