Screening and identification of proteins related to gastric cancer metastasis with comparative proteomics / 南方医科大学学报
Journal of Southern Medical University
; (12): 360-364, 2015.
Article
ي Zh
| WPRIM
| ID: wpr-239177
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen and identify the proteins related with tumor metastasis of gastric cancer in a nude mouse model bearing orthotopic transplanted tumor.</p><p><b>METHODS</b>Zinc finger protein 139 (ZNF139)-specific siRNA was synthesized and transfected into gastric cancer cell line SGC7901, which was then screened by G418. ZNF139-siRNA-transfected cells, negative plasmid-transfected cells and untreated SGC7901 cells were orthotopically transplanted separately on the stomach wall of BALB/c nude mice. The primary tumors and metastatic lymph nodes were harvested to separate the proteins by 2-D fluorescence difference gel electrophoresis (2-D DIGE); after gel digestion, the differential proteins were subjected to liquid chromatography-mass spectrometry (LC-MS) for identification and their functions were analyzed. Western blotting was performed to verify the identified proteins.</p><p><b>RESULTS</b>ZNF139 expression was effectively inhibited in siRNA-transfected SGC7901 cells. ZNF139-siRNA-transfected cells showed obviously suppressed tumor growth with a lowered lymph node metastasis rate in nude mice compared with untreated cells and the negative control cells (P<0.05). Proteomic study with 2-D DIGE showed that fascin and hnRNPA2/B1 were down-regulated while ANXA1 was up-regulated in the primary tumors, and ANXA5 was down-regulated in the metastatic lymph nodes in ZNF139-siRNA-transfected group. Western blotting confirmed the results of proteomic analysis.</p><p><b>CONCLUSION</b>ZNF139 gene may promote lymph node metastasis of gastric cancer by regulating fascin, hnRNPA2/B1, ANXA1, and ANXA5.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
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Stomach Neoplasms
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Transfection
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Electrophoresis, Gel, Two-Dimensional
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Blotting, Western
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Chromatography, Liquid
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Annexins
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B
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RNA, Small Interfering
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Proteomics
نوع الدراسة:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
المحددات:
Animals
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Humans
اللغة:
Zh
مجلة:
Journal of Southern Medical University
السنة:
2015
نوع:
Article