Chemical structure of fructosan from Condonopsis pilosula / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the polysaccharide of Condonopsis pilosula.</p><p><b>METHOD</b>The polysaccharide, CPP-1, was purified by DE-52 cellulose and Sephadex G-200 gel column chromatography. Purity and molecular weight of the polysaccharide were determined by gel permeation chromatography. Methylation analysis, periodate oxidation and degradation, IR, 1H-NMR and 13C-NMR methods were adopted to elucidate the chemical structure.</p><p><b>RESULT</b>The molecular weight of CPP-1 was estimated to be 7.5 x 10(4), and the structure of CPP-1 was a beta-(2 --> 1) linked beta-D-fructosan.</p><p><b>CONCLUSION</b>CPP-1 was a neutral homosaccharide.</p>