Development and analysis of a kinetic model for Escherichia coli threonine biosynthesis / 生物工程学报
Chinese Journal of Biotechnology
; (12): 18-29, 2014.
Article
ي Zh
| WPRIM
| ID: wpr-242416
المكتبة المسؤولة:
WPRO
ABSTRACT
Kinetic model analysis is a useful tool for understanding the regulation and control of cellular metabolism and thus offering a guideline for rational design of high efficiency cell factory. Based on previously published models and experimental measurement of enzyme kinetics data, we developed a kinetic model for the threonine biosynthesis pathway in Escherichia coli. This model integrates the central pathways that produce precursors, ATP and reducing power with the threonine biosynthesis pathway from aspartate. In contrast to the previous models, we considered the energy and reducing power balance rather than artificially set their concentrations. Metabolic control analysis of the model showed that enzymes PTS, G6PDH, HDH etc. have great flux control coefficients on the threonine biosynthesis flux. This indicates higher threonine synthesis flux could be achieved by overexpressing these enzymes.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Threonine
/
Industrial Microbiology
/
Kinetics
/
Escherichia coli
/
Metabolic Networks and Pathways
/
Metabolism
/
Models, Biological
نوع الدراسة:
Prognostic_studies
اللغة:
Zh
مجلة:
Chinese Journal of Biotechnology
السنة:
2014
نوع:
Article