Molecular epideiological and clinical feature of human calicivirus and adenovirus among children with diarrhea less than 5 years old from 2010 to 2011 in Lanzhou, Gansu province / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the clinical and molecular epidemiology characteristics of calicivirus and adenovirus in children for viral diarrhea in Lanzhou.</p><p><b>METHODS</b>Stool samples were collected from 295 children with diarrhea at the First Hospital of Lanzhou University, Gansu Province,China, between July 2010 and June 2011. Reverse transcription-polymerase chain reaction (RT-PCR) or PCR were used to detected calicivirus and adenovirus. The adenovirus positive samples were typed by nested PCR and multiple PCR.</p><p><b>RESULTS</b>Of the 295 specimens, 13.2% (39/295) were positive for calicivirus, and 5.1% (15/295) were adenovirus. Typing and Phylogenetic analysis revealed that novirus GII-3 and adenovirus 41 were the dominant strains. Both calicivirus and adenovirus predominately affect children under the age of 2. In seasonal distribution, there was no obvious peak.</p><p><b>CONCLUSION</b>Human calicivirus and adenovirus are important pathogens of viral diarrhea,it is important to develop long-term systematic surveillance.</p>