Effect of Tyrosine Phosphorylation Sites of Oncogenic Protein NPM-ALK on Cell Cycle and Its Related Mechanisms / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1201-1205, 2016.
Article
ي Zh
| WPRIM
| ID: wpr-246791
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>UNLABELLED</b>Objective:To explore the effect of tyrosine phosphorylation sites Tyr644 and Tyr664 in oncogenic protein NPM-ALK on cell cycle and its related mechanisms.</p><p><b>METHODS</b>Transiently transfected 293T cells and stably transfected Jurkat cells were used for analysis of cell cycle and protein after the transfection with the constructed recombinant plasmid pEGFP-N1, pEGFP-N1-NPM-ALK and pEGFP-N1-NPM-ALK(644, 664); soft agar assay for colony formation was performed to examine the different carcinogenicity of stable cell lines; cell viability of stable cell lines was examined by CCK-8 after the treatment with PPP.</p><p><b>RESULTS</b>The S arrest occurred in both NPM-ALK(644,664) transfected 293T and Jurkat cells; the susceptibility of NPM-ALK transfected Jurkat cells to PPP was highest among the 3 stable cell lines; the phosphorylated levels of AKT, ERK and STAT3 were decreased in NPM-ALK(644,664) cells compared with the NPM-ALK ones. Additionally, the double mutation induced the increase of CDK2 and the decrease of P27 (P<0.05).</p><p><b>CONCLUSION</b>The mutation of Tyr644 and 664 sites in NPM-ALK can induce cell cycle arrest in S phase and lower susceptibility to PPP that may be related with the phosphorylation change of cell growth related molecules in the downstream of NPM-ALK.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Oncogenes
/
Phosphorylation
/
Tyrosine
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Protein-Tyrosine Kinases
/
Transfection
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Signal Transduction
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Cell Cycle
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Cell Survival
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Jurkat Cells
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Cell Proliferation
المحددات:
Humans
اللغة:
Zh
مجلة:
Journal of Experimental Hematology
السنة:
2016
نوع:
Article